Data from: Genetic analyses in Lake Malawi cichlids identify new roles for Fgf signaling in scale shape variation

Description

Elasmoid scales are the most common epithelial appendage among vertebrates, however an understanding of the genetic mechanisms that underlie variation in scale shape is lacking. Using an F2 mapping cross between morphologically distinct cichlid species, we identified >40 QTL for scale shape at different body positions. We show that while certain regions of the genome regulate variation in multiple scales, most are specific to scales at distinct positions. This suggests a degree of regional modularity in scale development. We also identified a single QTL for variation in scale shape disparity across the body. Finally, we screened a QTL hotspot for candidate loci, and identified the Fgf receptor fgfr1b as a prime target. Quantitative rtPCR and small molecule manipulation support a role for Fgf signaling in shaping cichlid scales. While Fgfs have previously been implicated in scale loss, these data reveal new roles for the pathway in scale shape variation.,Parental_F2_Residuals_dataraw data for scale shape measures for parental species and a subset of F2 hybrids.LFxTRC_F2_scale_pheno_dataF2 phenotypes for QTL mapping. Genotypic data are under a different DOI.F2_scale1-6raw landmark data for all F2 hybrids in TPS format.LF_TRC_parental_scale1-6raw landmark data for parental scales in TPS format.SU5402_treatmentraw phenotypic data for scales post small molecule treatment.Cichlid_Scale_qPCR_DataqPCR data used to compare scale types between and within species.Parental_F2_sex_SLSex and standard length of fish used in this studyparental_F2scale_numberScale number in the fish used in this study.

Publication Date

1-1-2019

Publisher

DRYAD

DOI

10.5061/dryad.35cp405

Funder

National Science Foundation

Language

en

Document Type

Data Set

Identifier

10.5061/dryad.35cp405

Embargo Date

1-1-2019

Version

2

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