Transcriptomic profiling of tall fescue in response to heat stress and improved thermotolerance by melatonin and 24-epibrassinolide

Creators

Yang Liu, Hubei Academy of Agricultural Science, Institute of Poultry and Veterinarian
Zhulong Chan, Hubei Engineering University, Key Laboratory for Quality Control of Characteristic Fruits and Vegetables of Hubei Province, College of Life Science and Technology
Li Yang, University of Chinese Academy of Sciences
Hong Luo, Clemson University, Department of Genetics and Biochemistry
Lihua Zhang, Wuhan University, Institute for Advanced Studies and College of Life Science
Pingfang Yang, Chinese Academy of Sciences, Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden/Sino-Africa Joint Research Center
Mohammad Nur Alam, Bangladesh Agricultural Research Institute, Agronomy Division, Wheat Research Centre
Qingfeng Wang, Chinese Academy of Sciences, Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden/Sino-Africa Joint Research Center
Md.Rabiul Islam, University of Chinese Academy of Sciences

Description

Abstract Background Tall fescue is a widely used cool season turfgrass and relatively sensitive to high temperature. Chemical compounds like melatonin (MT) and 24-epibrassinolide (EBL) have been reported to improve plant heat stress tolerance effectively. Results In this study, we reported that MT and EBL pretreated tall fescue seedlings showed decreased reactive oxygen species (ROS), electrolyte leakage (EL) and malondialdehide (MDA), but increased chlorophyll (Chl), total protein and antioxidant enzyme activities under heat stress condition, resulting in improved plant growth. Transcriptomic profiling analysis showed that 4311 and 8395 unigenes were significantly changed after 2Â h and 12Â h of heat treatments, respectively. Among them, genes involved in heat stress responses, DNA, RNA and protein degradation, redox, energy metabolisms, and hormone metabolism pathways were highly induced after heat stress. Genes including FaHSFA3, FaAWPM and FaCYTC2 were significantly upregulated by both MT and EBL treatments, indicating that these genes might function as the putative target genes of MT and EBL. Conclusions These findings indicated that heat stress caused extensively transcriptomic reprogramming of tall fescue and exogenous application of MT and EBL effectively improved thermotolerance in tall fescue.

Publication Date

1-1-2018

Publisher

figshare Academic Research System

DOI

10.6084/m9.figshare.c.4047977.v1

Document Type

Data Set

Recommended Citation

Liu, Yang; Chan, Zhulong; Yang, Li; Luo, Hong; Zhang, Lihua; Yang, Pingfang; Alam, Mohammad Nur; Wang, Qingfeng; Islam, Md.Rabiul (2018), "Transcriptomic profiling of tall fescue in response to heat stress and improved thermotolerance by melatonin and 24-epibrassinolide", figshare Academic Research System, doi: 10.6084/m9.figshare.c.4047977.v1

https://doi.org/10.6084/m9.figshare.c.4047977.v1

Identifier

10.6084/m9.figshare.c.4047977.v1

Related Content

10.1186/s12864-018-4588-y

Embargo Date

1-1-2018