Additional file 6 of PA1 participates in the maintenance of blood–testis barrier integrity via cooperation with JUN in the Sertoli cells of mice
Description
Additional file 6: Fig. S4. Amh-pa1−/− mice show massive germ cell loss. a) Most of the residual cells in the empty tubules of Amh-pa1−/− testis were identified as Sertoli cells. Immunofluorescence analysis using anti-WT1 (green) was conducted on the sections of testis from Pa1F/F mice (upper panels) and Amh-pa1−/− mice (lower panels). Nuclei were stained with DAPI (blue). Dashed curve indicated basal membrane of seminiferous tubules. b) PCNA immunofluorescence analysis of seminiferous tubules showed that 8-week-old Amh-pa1−/− mice had decreased numbers of spermatocytes and spermatogonia compared with 8-week-old Pa1F/F mice. Seminiferous tubule sections of Pa1F/F and Amh-pa1−/− testes were stained for PCNA (red), and the nuclei was stained with DAPI (blue). Dashed boxes showed the localization of the enlarged images. Dashed curve indicated basal membrane of seminiferous tubules. Data are presented as mean ± SEM. ***p
Publication Date
1-1-2022
Publisher
figshare Academic Research System
DOI
10.6084/m9.figshare.19518630.v1
Document Type
Data Set
Recommended Citation
Liu, Bo; Li, Zhen; Zhao, Zhiwei; Cao, Wanjun; Xiao, Sai; Liu, Chao; Zhang, Ruidan; Ma, Yanjie; Li, Wei; Ma, Binfang; Zhu, Guozhang (2022), "Additional file 6 of PA1 participates in the maintenance of blood–testis barrier integrity via cooperation with JUN in the Sertoli cells of mice", figshare Academic Research System, doi: 10.6084/m9.figshare.19518630.v1
https://doi.org/10.6084/m9.figshare.19518630.v1
Identifier
10.6084/m9.figshare.19518630.v1
Embargo Date
1-1-2022