QTL mapping and marker development for tolerance to sulfur phytotoxicity in melon (Cucumis Melo)
Description
Elemental sulfur is an effective, inexpensive fungicide for many foliar pathogens, but severe phytotoxicity prohibits its use on many melon varieties. Sulfur phytotoxicity causes chlorosis and necrosis of leaf tissue, leading to plant death in the most sensitive lines, while other varieties have little to no damage. A high-density, genotyping-by-sequencing (GBS)-based genetic map of a recombinant inbred line (RIL) population segregating for sulfur tolerance was used for a quantitative trait loci (QTL) mapping study of sulfur phytotoxicity in melon. One major (qSulf-1) and two minor (qSulf-8 and qSulf-12) QTL were associated with sulfur tolerance in the population. The development of Kompetitive Allele-Specific PCR (KASP) markers developed across qSulf-1 decreased the QTL interval from 239 kb (cotyledons) and 157 kb (leaves) to 97 kb (both tissues). The markers were validated for linkage to sulfur tolerance in a set of melon cultivars. These KASP markers can be incorporated into melon breeding programs for introgression of sulfur tolerance into elite melon germplasm.
Publication Date
8-6-2020
Publisher
Zenodo
DOI
10.5061/dryad.zkh18937m
Document Type
Data Set
Recommended Citation
Branham, Sandra; Daley, James; Hassell, Richard; Wechter, Patrick; Levi, Amnon (2020), "QTL mapping and marker development for tolerance to sulfur phytotoxicity in melon (Cucumis Melo)", Zenodo, doi: 10.5061/dryad.zkh18937m
https://doi.org/10.5061/dryad.zkh18937m
Identifier
3975135
Embargo Date
8-6-2020
Version
1