Document Type

Patent

Publication Date

7-10-2001

Patent Number

patent number 6258537

Abstract

The present invention provides a sensitive test for objectively diagnosing the presence of Didymella bryoniae, the causative agent of gummy stem blight, and differentiating it from similar, nonpathogenic Phoma species. The assay is applicable to DNA isolated from extracts from plant leaves, stem or seed. The detection method employs a polymerase chain reaction technique, using specific oligonucleotide primers for amplification. PCR Products can be visualized using an ELISA-based colorimetric detection system.

Application Number

09/255432

Assignees

Clemson University (Clemson, SC)

Filing Date

1999-02-22

Primary/U.S. Class

435/6.12

Other/U.S. Class

435/91.2, 536/22.1, 536/24.3, 536/24.32, 536/25.32

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