Document Type
Patent
Publication Date
10-24-2017
Patent Number
9795573
CPC
A01N 1/0231 (20130101); A61K 31/17 (20130101); A61K 31/7028 (20130101); A61K 31/7036 (20130101); A61K 35/34 (20130101); A61K 45/06 (20130101); A61K 31/7036 (20130101); A61K 31/17 (20130101); A61K 31/7028 (20130101); A61K 31/15 (20130101); A61K 2300/00 (20130101); A61K 2300/00 (20130101); A61K 2300/00 (20130101)
Abstract
A multi-step stabilization method for connective tissue is described. Stabilized tissues can exhibit increased resistance to degradation due to enzyme activity, fatigue and storage. The multi-step method includes a first step during which the tissue can be incubated with a glycosaminoglycanase inhibitor such as a sulfated oligosaccharide, one example of which being neomycin, a second step during which the tissue can be incubated with a crosslink activator such as a carbodiimide crosslink activator and/or a crosslinking agent such as a heterobifunctional crosslinking agent and/or a phenolic compound such as a tannin, examples of which include tannic acid and pentagalloylglucose, and a third step during which the tissue can be incubated with a second crosslink activator that can be the same or different as the first crosslink activator.
Application Number
14/495,067
Assignees
Clemson University (Clemson, SC)
Filing Date
September 24, 2014