Document Type

Patent

Publication Date

10-24-2017

Patent Number

9795573

CPC

A01N 1/0231 (20130101); A61K 31/17 (20130101); A61K 31/7028 (20130101); A61K 31/7036 (20130101); A61K 35/34 (20130101); A61K 45/06 (20130101); A61K 31/7036 (20130101); A61K 31/17 (20130101); A61K 31/7028 (20130101); A61K 31/15 (20130101); A61K 2300/00 (20130101); A61K 2300/00 (20130101); A61K 2300/00 (20130101)

Abstract

A multi-step stabilization method for connective tissue is described. Stabilized tissues can exhibit increased resistance to degradation due to enzyme activity, fatigue and storage. The multi-step method includes a first step during which the tissue can be incubated with a glycosaminoglycanase inhibitor such as a sulfated oligosaccharide, one example of which being neomycin, a second step during which the tissue can be incubated with a crosslink activator such as a carbodiimide crosslink activator and/or a crosslinking agent such as a heterobifunctional crosslinking agent and/or a phenolic compound such as a tannin, examples of which include tannic acid and pentagalloylglucose, and a third step during which the tissue can be incubated with a second crosslink activator that can be the same or different as the first crosslink activator.

Application Number

14/495,067

Assignees

Clemson University (Clemson, SC)

Filing Date

September 24, 2014

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