Sequencing of a QTL-rich Region of the Theobroma cacao Genome using Pooled BACs and the Identification of Trait Specific Candidate Genes

Authors

Frank A. Feltus, Clemson University
Christopher Saski, Clemson UniversityFollow
Keithanne Mockaitis, Indiana University - Bloomington
Niina Haiminen, IBM T.J. Watson Research Center
Laxmi Parida, IBM T.J. Watson Research Center
Zachary Smith, Indiana University - Bloomington
James Ford, Indiana University - Bloomington
Margaret E. Staton, Clemson University
Stephen P. Ficklin, Clemson University
Barbara P. Blackmon, Clemson University
Chun-Huai Cheng, Clemson University
Raymond J. Schnell, Subtropical Horticulture Research Station, USDA-ARS
David N. Kuhn, Subtropical Horticulture Research Station, USDA-ARS
Juan-Carlos Motamayor, Mars Incorporated

Document Type

Article

Publication Date

7-2011

Publication Title

BMC Genomics

Publisher

BioMed Central

Abstract

BAC-based physical maps provide for sequencing across an entire genome or a selected sub-genomic region of biological interest. Such a region can be approached with next-generation whole-genome sequencing and assembly as if it were an independent small genome. Using the minimum tiling path as a guide, specific BAC clones representing the prioritized genomic interval are selected, pooled, and used to prepare a sequencing library.

Comments

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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