"Biofabrication and Evaluation, in vitro and in vivo, of a Dual Respons" by Olukayode Karunwi, Fouzan Alam et al.
 

Graduate Research and Discovery Symposium (GRADS)

Document Type

Poster

Publication Date

Spring 2015

Abstract

With the current state of progress in trauma management by first responders, there is a need for development of an electrochemical biotransducer that can be used with a wireless implantable biosensor system for continual measurement of interstitial glucose and lactate; beginning from the site of the accident and en-route to a trauma center. Fabrication of oxidoreductase enzyme-rich biorecognition membranes deposited via pyrrole electropolymerization at microfabricated electrodes has been achieved. This construct was then electrochemically overoxidzed to create a non-conductive enzyme-hosting polymer film and finally the entire biotransducer was coated with a phosphorylcholine-containing biomimetic hydrogel to mitigate biofouling and reduce the foreign body response. A catalytic layer of Ni-hexacyanoferrate placed at the electrode-enzyme interface for enhanced peroxide response produced a 20-fold increase (14.19 nA vs. 0.7 nA) in buffered H2O2 measured at 650 mV vs. Ag/AgCl. In vitro characterization showed a sensitivity of 0.68 mA/cm2/mM and 0.36 mA/cm2/mM and a limit of detection of 0.05 mM and 7.9 mM for glucose and lactate respectively.

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