Graduate Research and Discovery Symposium (GRADS)

Document Type

Poster

Publication Date

Spring 2015

Abstract

Capillary-channeled polymer (C-CP) fibers have been under investigation and development as stationary phase for high performance liquid chromatography (HPLC) separations for over ten years. They have eight capillary channels extending the whole length of the fibers. This unique shape offers them ~3x greater surface area than that of circular cross-section fibers with the same nominal diameter. When packed into a column, C-CP fibers self-align, yielding a monolith like structure of 1-5 µm open, parallel channels. As a result, C-CP fiber stationary phases exhibit excellent fluid transport properties. In this study, a polyethylene terephthalate (PET) C-CP fiber packed microbore HPLC column was sequentially functionalized. The common biotin-streptavidin affinity pair was used as a demonstration of the basic methodology, wherein biotin was the immobilized ligand. The biotin functionalized PET C-CP fiber column was shown to be able to affinity capture streptavidin from an enhanced green fluorescence protein (EGFP)-spiked E. coli cell lysate with high selectivity and efficiency. The coupling method used here yields PET C-CP fiber stationary phases with great potential for further functionalization by a variety of ligands and used for different applications.

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