Date of Award
5-2015
Document Type
Dissertation
Degree Name
Doctor of Philosophy (PhD)
Legacy Department
Biological Sciences
Committee Chair/Advisor
Yanzhang Wei
Committee Member
Lisa Bain
Committee Member
Charles Rice
Committee Member
Xianzhong Yu
Abstract
One of the characteristics of advanced tumors is the evasion of the immune system. There are multiple methods that tumor cells employ to achieve this including reducing the expression of activating ligands on the cell surface and a shift in the tumor microenvironment toward pro-tumor cytokines. The purpose of this research is to develop a novel bifunctional fusion protein that will target these two deficiencies in the tumor microenvironment and activate killer cells that are already present. The proposed protein combines the extracellular domain of a ligand for the killer cell activating receptor NKG2D and Interleukin-12 (IL-12). It is hypothesized that when expressed by tumor cells by gene therapy, the protein will simultaneously activate NK and other killer cells using the NKG2D receptor, and deliver a locally high dose of IL-12 to the tumor microenvironment where it can interact with the IL-12 receptor and enhance cytotoxicity.
Recommended Citation
Tietje, Ashlee, "Killer cell activation by a novel multifunctional protein: an immuno/gene therapy for cancer" (2015). All Dissertations. 1520.
https://open.clemson.edu/all_dissertations/1520
Included in
Biology Commons, Medical Immunology Commons, Oncology Commons