Date of Award

12-2025

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Forest Resources

Committee Chair/Advisor

Dr. David R Coyle

Committee Member

Dr. R. Talbot Trotter, III

Committee Member

Dr. Muhammad Z. Ahmed

Abstract

The Asian longhorned beetle (Anoplophora glabripennis Motschulsky; ALB) is a major threat to forest health due to the damage done by larval feeding in host trees. In 2020, ALB was discovered in Charleston County, South Carolina, which presents a novel climate for this invasive pest in North America. I investigated the reproductive biology and larval development of ALB to support detection and management efforts. By exposing 19 host trees to mated pairs of adults, I was able to determine rates of oviposition success (55%), egg viability (55%), and early larval survival (56%). On average, females chewed nearly 1 egg site per beetle per day; however, oviposition rates were variable among individuals. I also examined larval morphometrics, specifically head capsule width and dry weight, and larval feeding behavior by sampling 1,351 larvae across >3 generations. Seasonal changes in larval morphometrics indicated that ALB is univoltine in South Carolina and maintains a diapause period over the winter; although variation in these data suggests a subgroup of larvae may develop at a slightly faster or slower rate. I also identified the temporal and morphological trends associated with the larval transition from phloem to xylem feeding. Most of the phloem-feeding larvae were 1st, 2nd, and 3rd instar, with the feeding transition occurring in the 3rd or 4th instar. Improving our understanding of ALB reproduction and larval development in a novel environment benefits risk analysis, survey, and life-stage dependent management techniques.

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