Date of Award

5-2026

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Genetics and Biochemistry

Committee Chair/Advisor

Kelsey Witt Dillon

Committee Member

James Lewis

Committee Member

Shyamalika Gopalan

Committee Member

Alex Feltus

Abstract

The peopling of the Americas created new opportunities for genetic adaptation as ancient populations encountered novel environments across North and South America. Previous research has shown that segments of Indigenous American ancestry contain elevated proportions of Denisovan alleles. The gene CNTNAP2, which is involved in brain development and neuronal function, contains a Denisovan-derived haplotype that represents a candidate locus for adaptive introgression in admixed American populations. A Denisovan-derived core haplotype approximately 55 kb in length was identified within intron 13 of CNTNAP2. This haplotype is primarily found in admixed American populations and occurs rarely outside the Americas, and typically in a heterozygous state. Screening of ancient genomes identified the haplotype in multiple individuals predating European colonization, indicating persistence through time in pre-contact populations. The introgressed region overlaps a single annotated feature, a long noncoding RNA of unknown function that shows expression in brain tissues. Additional scans for selection produced signals consistent with the maintenance of this haplotype in Indigenous American populations. These results indicate that a Denisovan-derived haplotype at CNTNAP2 persisted in Indigenous American populations and represents a candidate locus for archaic adaptive introgression in the Americas.

Included in

Genomics Commons

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