Date of Award
5-2026
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Bioengineering
Committee Chair/Advisor
Bruce Z. Gao
Committee Member
Dan Simionescu
Committee Member
Agneta Simionescu
Abstract
We developed an integrated bioprinting and perfusion platform to generate vascularized hydrogel constructs that support perfusable microchannel networks. Multiple hydrogel formulations, sacrificial ink systems, device geometries, and perfusion conditions were systematically evaluated to optimize print fidelity, mechanical stability, and cellular compatibility. Internally gelling alginate–calcium carbonate hydrogels were identified as a robust matrix, providing tunable gelation kinetics and structural stability during embedded printing. In parallel, Pluronic F-127 fugitive ink enabled reliable fabrication of continuous hollow channels due to its shear-thinning behavior and efficient post-print removal.
The finalized platform integrates stereolithographically fabricated perfusion chambers with extrusion-based bioprinting to produce three-dimensional, perfusable vascular architectures. Rheological characterization defined a transient printing window during gelation that supports embedded deposition while allowing localized crosslinking. This approach enables the formation of stable channels that sustain fluid flow and support endothelial cell attachment.
This work addresses a key limitation in therapeutic development, where preclinical models often fail to accurately predict human drug responses, contributing to high failure rates, increased costs, and delayed patient access to new treatments. By enabling controlled perfusion and cellular integration, this platform advances the development of vascularized microphysiological systems for in vitro drug screening and reduces reliance on animal testing.
Recommended Citation
Chernyatinskiy, Calvin A., "Design and Development of a Perfusable Hydrogel Platform With Bioprinted Human-Endothelialized Microvascular Channels" (2026). All Theses. 4769.
https://open.clemson.edu/all_theses/4769