Date of Award
12-2009
Document Type
Thesis
Degree Name
Master of Science (MS)
Legacy Department
Microbiology
Committee Chair/Advisor
Scott, Thomas
Committee Member
Rice , Charles
Committee Member
Wei , Yanzhang
Committee Member
Tzeng , Tzuenrong
Abstract
This study was conducted to determine if the p38 and ERK1/2 MAPK pathways are involved in transcription of IL-6, IL-8, and COX-2 in the chicken thrombocyte response to ligation of Toll-like receptor 4 (TLR4) by Salmonella minnesota lipopolysaccharide (LPS). Thrombocytes were isolated and subsequently treated with either p38 MAPK or ERK inhibitor, and then with LPS during in vitro cell culture. Transcription of IL-6, IL-8, and COX-2 mRNA was determined using real-time PCR. The experiments were repeated using pM, nM and μM concentrations of both inhibitors to test cell sensitivity. Stimulation with LPS induced expression of IL-6, IL-8 and COX-2 mRNA. This expression was reduced by treatment with 25 μM ERK inhibitor as well as by treatment with 70 μM p38 MAPK inhibitor. However, treatment with 25 pM and nM ERK inhibitor, and with 70 pM and nM p38 MAPK inhibitor, yielded no decrease in mRNA expression in relation to treatment with LPS alone. Inhibition with both the ERK and p38 MAPK inhibitors was in accordance with previously established concentrations for mammalian cells. It is now apparent that the p38 and ERK1/2 MAPK pathways are both linked to gene expression of a cytokine, a chemokine and an enzyme involved in the thrombocyte innate response to TLR4 ligation by bacterial LPS.
Recommended Citation
Hitchcock, Candace, "ERK 1/2 AND p38 MAPK PATHWAYS ARE BOTH INVOVLED IN THE EXPRESSION OF INTERLEUKIN-6, -8, AND CYCLOOXYGENASE-2 IN THROMBOCYTES STIMULATED WITH LIPOPOLYSACCHARIDE" (2009). All Theses. 701.
https://open.clemson.edu/all_theses/701