Date of Award
5-2010
Document Type
Thesis
Degree Name
Master of Science (MS)
Legacy Department
Chemistry
Committee Chair/Advisor
Harcum, Sarah W
Committee Member
Christensen , Kenneth
Committee Member
Chumanov , George
Abstract
High quality, intact RNA is required for DNA microarray studies, cloning, and reverse transcriptase polymerase chain reaction (rt-PCR) analysis. There are several analytical methods used to assess the RNA quality. The RNA Integrity Number (RIN) from the Agilent Bioanalyzer is one quality control assay used to evaluate RNA. For recombinant Escherichia coli cultured under stressful conditions the RNA profiles obtained using the Agilent Bioanalyzer indicate RNA degradation; however, RNA obtained and purified in parallel from unstressed recombinant cultures indicate acceptable RNA values without significant degradation. We proposed that for stressed E. coli the RIN value is not necessarily indicative of RNA purification-related degradation but could be used as a tool to characterize and detect stressful culture conditions that target ribosomal RNA. Also, these results suggest the need for caution when assessing RNA quality based on ribosomal RNA abundance in stressed cells.
Recommended Citation
Salazar, Mary alice, "COMPARISON OF RNA QUALITY FROM STRESSED AND UNSTRESSED RECOMBINANT ESCHERICHIA COLI" (2010). All Theses. 768.
https://open.clemson.edu/all_theses/768